## Description of the "Temperature Scan with Changer for Decay Measurement" script

### Short explanation of operation:

The script increases the temperature of the sample holder in steps, and measures a decay histogram for each temperature. A delay can be introduced after reaching the set temperature of the sample holder to ensure optimal temperature equilibrium also in the cuvette. A simple intensity optimizer ensures that the given pile up ratio is not violated.

The sample changer is used to measure an instrument response function for each temperature.

### Hardware prerequisites:

FluoTime300 with

- Sample Changer Turret 400 (4 position cuvette holder with temperature control)
- Detection polarizer
- DETECTOR_1 (used for measurement)

Note: It is possible to use use DETECTOR_2 for the measurement. Replace all occurrences of DETECTOR_1 in the script by DETECTOR_2

### Software prerequisites:

EasyTau Version > 1.1

### Prior running the script

Please adapt the script to your needs

#### Please set manually:

Emission monochromator exit port to deliver light to DETECTOR_1
- Emission monochromator slit width / bandwidth to define the detection bandpass
- Electrical switches (if exist) to deliver electrical signal from DETECTOR_1 to TCSPC electronics
- Laser settings wavelength, repetition frequency, intensity
- TCSPC electronics for measurement
- TCSPC electronics bin width

#### Values to adjust in the script:

- Sample: Sample name for workspace
- Solvent: Solvent name for workspace
- SamplePosition: Position of sample in the sample changer
- SamplePosition: Detection wavelength given in nanometer
- ScatterPosition: Position of scatter solution in the sample changer
- IRFDetectionWL: Laser IRF wavelength given in nanometer
- StartTemp: Start of temperature ramp in °C
- StopTemp: End of temperature ramp in °C
- TempStep: Step size for temperature ramp in °C
- EqDelay: Delay in seconds to wait for temp. equilibrium in the sample